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采用低浓度非离子型去污剂ＴｒｉｔｏｎＸ－１１４（ＴＸ－１１４）抽提和分离问号状钩端螺旋体（钩体）黄疸出血群赖型０１７株（０１７），选择性地将钩体外膜蛋白分离为ＴＸ－１１４疏水相和亲水相两个部分。疏水相外膜蛋白含５条主要蛋白区带，其分子量分别为６６ｋｄ、３９ｋｄ、３５ｋｄ、２７ｋｄ和１６ｋｄ，其中仅３９ｋｄ外膜蛋白区带可特异地分别被抗全０１７血清、抗０１７外膜血清和抗０１７保护性单克隆抗体Ｅ４Ｂ７Ｇ５所识别。本研究结果表明经ＴＸ－１１４抽提分离的３９ｋｄ疏水外膜蛋白为０１７钩体稳定的免疫原，在研究钩体保护性抗原和构建基因工程疫苗中有十分重要的研究价值。
The Leptospira interrogans (Leptospira interrogans) strain 017 (017) was isolated and isolated from Triton X-114 (TX-114), a low concentration nonionic detergent. For the TX-114 hydrophobic phase and hydrophilic phase in two parts. The hydrophobic outer membrane protein contained five major protein bands with molecular weights of 66kd, 39kd, 35kd, 27kd and 16kd, respectively, of which only the 39kd outer membrane protein band could be specifically blocked by anti-017 serum, anti-017 outer membrane serum And anti-017 protective monoclonal antibody E4B7G5. The results of this study indicate that the 39kd hydrophobic outer membrane protein extracted by TX-114 extraction is a 017 leptospira stable immunogen, which is of great value in the study of protective antigen of leptospira and construction of genetic engineering vaccine.