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目的探讨淫羊藿苷抑制骨吸收的机理。方法贴壁分离法培养大鼠骨髓基质干细胞,流式检测细胞纯度。加入10-5M淫羊藿苷12 h、24 h、36 h、48 h后用Real-time-RT-PCR检测OPG和RANKL基因表达,Western blotting检测蛋白表达。结果第2代培养细胞的纯度达到95%以上。OPG基因和蛋白表达水平在24 h时明显升高,与对照相比有显著差异。OPG和RANKL的比值显著高于对照组。结论淫羊藿苷主要通过提高OPG及OPG和RANKL的比值来发挥抑制骨吸收的作用。
Objective To investigate the mechanism of icariin inhibiting bone resorption. Methods Rat bone marrow stromal cells (MSCs) were cultured by adherent separation method and the cell purity was detected by flow cytometry. The expression of OPG and RANKL was detected by Real-time-RT-PCR after adding 10-5M icariin for 12 h, 24 h, 36 h and 48 h, respectively. Western blotting was used to detect the protein expression. Results The purity of the second generation of cultured cells reached more than 95%. OPG gene and protein expression levels significantly increased at 24 h, compared with the control there were significant differences. The ratio of OPG and RANKL was significantly higher than the control group. Conclusion Icariin inhibits bone resorption mainly by increasing the ratio of OPG, OPG and RANKL.