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为了深入探讨钙在心肌缺氧及复氧损伤中的作用,本工作采用Fura-2荧光测钙技术,观测了分离的成年大鼠心肌细胞在缺氧及复氧过程中胞浆游离钙[Ca~(2+)]_i的变化。用酶法分离大鼠心肌细胞,然后Fura-2/AM负载。
In order to further explore the role of calcium in myocardial hypoxia and reoxygenation injury, Fura-2 fluorescence calcium measurement was used to observe the changes of cytosolic free calcium [Ca ~ (2 +)] _ i. Rat cardiomyocytes were isolated enzymatically and then loaded on Fura-2 / AM.