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目的为进一步证实缺血、缺氧时神经原性ＮＯ的神经毒性作用。方法在大鼠离体海马脑片上，应用微电极记录技术，研究ｎＮＯＳ抑制剂７－ＮＩ对缺氧所致神经元损害的保护作用。结果ｎＮＯＳ抑制剂７－ＮＩ对海马脑片缺氧后群峰电位（ＰＳ）变化较对照组显著减小。结论７－ＮＩ对海马脑片缺氧损伤有明确保护作用。作用机理可能是７－ＮＩ抑制ｎＮＯＳ活性降低缺氧后海马神经原性ＮＯ的过多形成，从而提高海马脑片抗缺氧损伤的能力，更加明确了缺氧早期神经原性ＮＯ的神经毒性作用。
The purpose is to further confirm the neurotoxic effects of neurogenic NO during ischemia and hypoxia. Methods In vitro rat hippocampal slices, the application of microelectrode recording technology to study the protective effects of nNOS inhibitor 7-NI on hypoxic-induced neuronal damage. Results The nNOS inhibitor 7-NI significantly decreased the peak potential (PS) of hippocampal slices compared with the control group. Conclusion 7-NI has a clear protective effect on hippocampal slices of hypoxic injury. The possible mechanism is that 7-NI inhibits nNOS activity and reduces the excessive formation of neuronal NO in hippocampus after hypoxia, thus enhancing the ability of hippocampal slices to resist hypoxic damage and further clarifying the neurotoxic effect of early neurogenic NO in hypoxia .