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目的 :观察天麻钩藤方 (天钩方 )对不完全脑缺血清醒自由活动大鼠海马细胞外液递质氨基酸的影响。方法 :用CMA微透析采样系统收集双侧颈总动脉结扎和放松引起的清醒自由活动大鼠不完全缺血前、缺血期间及再灌后大鼠海马细胞外透析液 ;用高效液相色谱 电化学法测定透析液中谷氨酸 (Glu)、牛磺酸 (Tau)、精氨酸(Arg)、γ 氨基丁酸 (GABA)和半胱氨酸 (Cys)的浓度。结果 :天钩方中剂量组可升高正常大鼠脑海马细胞外液GABA和Tau的浓度。与模型组比较 ,大鼠不完全缺血 12 0min时 ,天钩方中和大剂量组的Glu水平分别降低了 38 6 4 %和31 35 % ,Tau升高了 13 99%和 12 86 % ,GABA升高了 2 5 89%和 33 99% ,Cys降低了 4 0 93%和 4 2 0 8% ,Arg升高了16 95 %和 8 96 % ;再灌 12 0min时 ,天钩方中和大剂量组的Glu的水平分别降低了 14 5 5 %和 11 4 8% ,Tau升高了16 13%和 14 0 3% ,GABA升高了 2 4 4 1%和 2 6 2 2 %。结论 :天钩方能增加正常大鼠海马细胞外液中抑制性氨基酸的浓度 ,抑制缺血再灌引起的兴奋性氨基酸的过度释放 ,增加Arg和抑制性氨基酸的浓度 ,从而发挥神经保护作用
Objective : To observe the effects of Tianma Quteng Decoction (Tiangou Fang) on hippocampal extracellular fluid-transporter amino acids in autonomic cerebral ischemia free-motion rats. METHODS: CMA microdialysis sampling system was used to collect extracellular dialysate in hippocampus of rats with conscious free movement induced by ligation and relaxation of common carotid artery before, during and after reperfusion. The concentration of glutamic acid (Glu), taurine (Tau), arginine (Arg), gamma amino butyric acid (GABA) and cysteine (Cys) in the dialysate was determined electrochemically. RESULTS: The middle-dose group of Tiangou prescription can increase the concentration of GABA and Tau in the extracellular fluid of hippocampus in normal rats. Compared with the model group, when the rats were not completely ischemic at 120 min, the Glu levels in the Tiangoufang middle- and high-dose group were reduced by 386.4% and 31.35 % respectively, and the Tau levels were increased by 1399% and 128%. GABA increased by 2859% and 33 99%, Cys decreased by 40% 93% and 40.28%, Arg increased by 169% and 896%; when reperfusion was 12 0min, Tiangou Fang Zhonghe The levels of Glu in high-dose groups decreased 145% and 114.8% respectively, Tau increased 1613% and 140.3%, and GABA increased 2441% and 2622%. Conclusion: Tiangou prescription can increase the concentration of inhibitory amino acids in extracellular fluid of hippocampus in normal rats, inhibit the excessive release of excitatory amino acids caused by ischemia and reperfusion, increase the concentration of Arg and inhibitory amino acids, and thus exert neuroprotective effect.