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目的：探索靶向性非病毒载体系统在人脑胶质瘤基因治疗中的应用。方法：组建了ＥＧＦ－Ｒ介导的ＧＥ７基因转移系统，分别与β－ｇａｌ报道基因和ｐ２１基因构成载体复合物，体外转染Ｕ２５１细胞，通过Ｘ－ｇａｌ染色、Ｗｅｓｔｅｒｎ ｂｌｏｔ分析、原位末端标记、ＤＮＡ梯带检测等，观察了外源基因的导入及对肿瘤细胞的抑制作用。结果：Ｘ－ｇａｌ染色表明，外源基因的导人率可达８０％；转染ｐ２１基因后，细胞生长受到明显抑制，第７天生长抑制率约５８％。原位末端标记及 ＤＮＡ梯带检测发现，转染细胞有明显的凋亡发生。结论：ＧＥ７载体系统能高效靶向地将外源基因导入肿瘤细胞，外源基因的表达可明显抑制肿瘤细胞的生长，有效诱导其凋亡。
Objective: To explore the application of targeted non-viral vector system in the gene therapy of human glioma. METHODS: The EGF-R-mediated GE7 gene transfer system was established and the vector was constructed with β-gal reporter gene and p21 gene respectively and transfected into U251 cells in vitro. The results of X-gal staining and Western blot analysis showed that in situ end-labeling , DNA ladder detection, observed the introduction of foreign genes and the inhibition of tumor cells. Results: The results of X-gal staining showed that the introduction rate of exogenous gene was up to 80%. After transfection with p21 gene, the cell growth was significantly inhibited and the growth inhibition rate was about 58% on the 7th day. In situ end labeling and DNA ladder detection showed that the transfected cells have obvious apoptosis. CONCLUSION: GE7 vector system can target foreign genes into tumor cells efficiently and efficiently. The expression of foreign genes can significantly inhibit the growth of tumor cells and induce apoptosis effectively.