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邻苯二甲酸酯是广泛用于合成多聚物的增塑剂,并大量排入环境中。本文通过体内和体外实验研究邻苯二甲酸酯对线粒体呼吸功能的影响。选35日龄的雄性Wistar大鼠,每5只为一组。体外实验中,将大鼠断头处死,立即取出睾丸,去被膜并用冰冷缓冲液(pH 7.4,含0.225 M甘露醇和10 mM Tris-HCl)制成匀浆,分离线粒体,调节线粒体悬液的蛋白质浓度为100 mg/ml,以结晶牛血清蛋白为标准,用Bradford法测定蛋白质。线粒体的耗氧量用Gavani型氧电极在混合培养液中测定。将邻苯二甲酸酯的二甲基亚砜(DMSO)溶液,在加底物前加入线粒体样品中,32℃培养两分钟,
Phthalates are plasticizers that are widely used in the synthesis of polymers and are released into the environment in large quantities. In this paper, in vivo and in vitro experiments to study the impact of phthalates on mitochondrial respiratory function. 35-day-old male Wistar rats, one for every 5 rats. In in vitro experiments, rats were decapitated and testis removed immediately, removed from the testis and homogenized in ice-cold buffer (pH 7.4, containing 0.225 M mannitol and 10 mM Tris-HCl) to separate mitochondria and regulate the mitochondrial suspension protein The concentration of 100 mg / ml, with crystalline bovine serum albumin as standard, using Bradford assay protein. Mitochondrial oxygen consumption was measured in a mixed medium using a Gavani-type oxygen electrode. The phthalate solution in dimethylsulfoxide (DMSO) was added to the mitochondrial sample before addition of the substrate and incubated at 32 ° C for two minutes.