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应用细胞培养、３Ｈ－ＴｄＲ和３Ｈ－Ｌｅｕｃｉｎｅ掺入方法，观察血小板生长因子ＢＢ（Ｐｌａｔｅｌｅｔ－ｄｅｒｉｖｅｄＧｒｏｗｔｈＦａｃｔｏｒＢＢ）对体外培养兔肺动脉平滑肌细胞ＤＮＡ和蛋白质合成的影响。结果表明：（１）当ＰＤＧＦ－ＢＢ浓度为１０ｎｇ／ｍｌ时，３Ｈ－ＴｄＲ掺入值已较对照组显著增高（６２６２．５±４１２．９ｖｓ８３３．５±１２４．０，Ｐ＜０．０５）；当ＰＤＧＦ－ＢＢ浓度为２０ｎｇ／ｍｌ时，３Ｈ－Ｌｅｕｃｉｎｅ掺入值亦较对照线显著增高（１０２１２．８±６３８．３ｖｓ７３４０．３±１１９７．９，Ｐ＜０．０５）。（２）ＰＤＧＦ－ＢＢ浓度在５－２５ｎｇ／ｍｌ范围内，３Ｈ－ＴｄＲ，３Ｈ－Ｌｅｕｃｉｎｅ掺入值与剂量直线相关（ｒＤＮＡ＝０．９７，ｒｐｒｏｔ＝０．９０Ｐ＜０．０５）。说明ＰＤＧＦ－ＢＢ刺激体外培养兔肺动脉平滑肌细胞ＤＮＡ和蛋白质合成。
Cell culture, 3H-TdR and 3H-Leucine incorporation method were used to observe the effect of platelet-derived growth factor (BBB) on the DNA and protein synthesis of rabbit pulmonary artery smooth muscle cells. The results showed that: (1) 3H-TdR incorporation was significantly higher than that of the control group (6262.5 ± 412.9 vs833.5 ± 124.0, P <0.05) when the concentration of PDGF-BB was 10ng / ; When the concentration of PDGF-BB was 20ng / ml, 3H-Leucine incorporation was significantly higher than the control (10212.8 ± 638.3vs7340.3 ± 1197.9, P <0.05). (2) The concentration of PDGF-BB was in the range of 5-25ng / ml. The values of 3H-TdR and 3H-Leucine were linearly correlated with the dosage (rDNA = 0.97, rprot = 0.90P <0.05). PDGF-BB stimulated DNA and protein synthesis in rabbit pulmonary artery smooth muscle cells in vitro.