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赤霉素或环剥处理分别对暗柳橙的花芽分化起阻遏或促进怍用。赤霉素处理使芽内总游离氨基酸,特别是碱性氨基酸含量明显下降。环剥处理则使上述氨基酸含量增多。赤霉素处理,绝大多数种类氨基酸减少,尤其是组氨酸、精氨酸、酪氨酸等。环剥处理,相当种类的氨基酸增多,尤其是苏氨酸、组氨酸、谷氨酸等。氨基酸变化可能与芽内合成特异蛋白质有关,与基因的表达与阻遏有关。但也可能是营养芽或生殖芽两者本身在代谢上的差异。本研究为进一步阐明花芽分化的实质原因提供了生物化学对比性背景资料。
Gibberellic acid or ring peel treatment respectively dark orange flower bud differentiation or promote use. Gibberellin treatment of total bud free amino acids, especially basic amino acid content decreased significantly. Girdling makes the above amino acid content increased. Gibberellin treatment, the vast majority of amino acids to reduce, especially histidine, arginine, tyrosine and so on. Girdling treatment, a considerable increase in the number of amino acids, especially threonine, histidine, glutamic acid and so on. Amino acid changes may be related to the synthesis of specific proteins in shoots, and gene expression and repression. However, there may also be metabolic differences between vegetative and reproductive buds themselves. This study provides biochemical contrasting background information for further clarifying the essential causes of flower bud differentiation.