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提出利用CO2激光器快速制作微阵列点样芯片。通过测量激光在不同功率下加工小孔或是对同一小孔加工不同次数时所得小孔孔径和深度,以此来确定激光器的各项性能参数。并且测得不同功率的激光穿透小孔以及穿透次数不同所得小孔孔径。最后利用直径约为79μm的10×10阵列式孔道制作微阵列点样芯片,于尼龙膜上点样,所得样点平均直径为200μm,并测得小孔孔径与样点两者相对标准偏差分别为4.3%和7.4%。
It is proposed to use CO2 lasers to make microarray spotting chips quickly. By measuring the laser at different power processing holes or the same hole processing different times the number of holes obtained when the aperture and depth, in order to determine the laser performance parameters. And measured the different power of the laser penetration of small holes and the number of holes obtained through different times the aperture. Finally, a 10 × 10 arrayed channel with a diameter of about 79 μm was used to fabricate a microarray spotting chip. The sample was spotted on a nylon membrane and the average diameter of the sample was 200 μm. The relative standard deviations 4.3% and 7.4%.