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利用了氨基乙硫醇在金电极表面形成自组装单分子膜，然后用水溶性的碳化二亚胺作为偶联活化剂，ｓＤＮＡ的５’端磷酸基与电极表面自组装膜上的氨基以磷酸氨基酯键的形式共价结合，从而在金电极表面形成ｓｓＤＮＡ单分子层。采用反射红外光谱对电极表面ｓＤＮＡ单分子层进行了表征，并用氧化还原电对Ｃｏ（ｂｐｙ）３＋／２＋３作为杂交指示剂对电极表面的ｓＤＮＡ层的响应特性进行了研究。
Aminoethanethiol is used to form a self-assembled monolayer on the surface of the gold electrode, and then the water-soluble carbodiimide is used as a coupling activator. The 5 ’terminal phosphate group of the sDNA and the amino group on the self- The form of the ester bond covalently binds to form a ssDNA monolayer on the gold electrode surface. Reflecting infrared spectroscopy was used to characterize the monolayer of sDNA on the surface of the electrode, and the response characteristics of the sDNA layer on the electrode surface were investigated by using redox couple Co (bpy) 3 + / 2 + 3 as hybridization indicator.