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为监测鲤春病毒血症(SVC)在北京地区的流行现状和特征,2007~2015年,从北京市9个区县采集673份样品,使用细胞分离法和聚合酶链式反应(PCR)进行检测,分离到的SVC病毒株,扩增糖蛋白部分基因片段,进行测序和做进化树分析。结果显示,2007~2015年北京市SVCV以散在性流行为主;发现28个SVCV分离株,阳性检出率为4.16%;28个SVCV分离株与美国的SVC分离株和在中国其它地方的SVC分离株在进化方向上一致,而与欧洲的SVC分离株明显不同。为预防控制SVC的发生和流行,应加强对水产苗种的管理,认真开展疫情监测与调查研究工作。
To monitor the prevalence and characteristics of the prevalence of carp virus viremia (SVC) in Beijing, 673 samples were collected from 9 districts and counties in Beijing from 2007 to 2015 and were isolated by cell isolation and polymerase chain reaction (PCR) Detection and isolation of the SVC virus strain, amplification of glycoprotein partial gene fragments, sequencing and phylogenetic tree analysis. The results showed that there were 28 SVCV isolates in Beijing in 2007-2015, with a prevalence of 4.16%. 28 SVCV isolates were associated with SVC isolates in the United States and SVC in other parts of China The isolates were evolutionarily identical but distinctly different from the European SVC isolates. In order to prevent and control the occurrence and prevalence of SVC, the management of aquatic fingerlings should be strengthened and careful monitoring and investigation of the epidemic should be carried out.