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利用不同浓度甲基磺酸乙酯(EMS)诱变大白菜自交系‘A03’种子,根据M1代植株发芽率、成活率、育性及M2代成活率,筛选出适宜的EMS诱变浓度为0.4%。采取EMS种子诱变1次、EMS种子诱变2次及EMS花蕾诱变结合小孢子培养的方法构建了含有4 253个家系及相应自交M2代种子的大白菜突变体库。在该突变体库M2群体中分别对苗期6 404株幼苗及莲座期7 756个单株的叶片性状进行了形态学调查,总的表型变异频率高达37.62%和26.33%。
The inbred lines ’A03’ of Chinese cabbage were mutagenized with ethyl methylsulfonate (EMS) at different concentrations. According to the germination rate, survival rate, fertility and M2 generation survival rate of M1 plants, the suitable EMS mutagenesis concentration Is 0.4%. A Chinese cabbage mutant library containing 4 253 pedigrees and corresponding selfing M2 progenies was constructed by EMS seed mutagenesis once, EMS seed mutagenesis twice and EMS flower bud mutation combined with microspore culture. Morphological investigation was conducted on the leaf traits of 6 404 seedlings at seedling stage and 7 756 at seedling stage in the M2 population of this mutant. The total phenotypic variation frequency was 37.62% and 26.33%, respectively.