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目的:观察氯胺酮对谷氨酸诱导大鼠脊髓背角星形胶质细胞凋亡和胞内游离钙浓度([Ca2+]i)变化的影响。方法:取新生2~3 d W istar大鼠T11~L6脊髓背角星形胶质细胞,原代纯化培养。将细胞随机分为:对照组(C组),谷氨酸组(G组),氯胺酮组(K组),余下3组为谷氨酸+不同浓度氯胺酮组(标记为GK1~GK3组),培养30 m in后取各细胞检测[Ca2+]i,再培养48 h检测星形胶质细胞凋亡率。结果:与C组比较,G组细胞发生了大量凋亡(P<0.01),[Ca2+]i显著升高(P<0.01);与G组比较,GK2组细胞凋亡率和[Ca2+]i明显降低(P<0.05),GK3组细胞凋亡率和[Ca2+]i显著降低(P<0.01)。结论:适量氯胺酮通过抑制细胞内钙超载显著抑制了谷氨酸诱导星形胶质细胞凋亡。
Objective: To investigate the effects of ketamine on the changes of astrocyte apoptosis and intracellular free calcium concentration ([Ca2 +] i) induced by glutamate in spinal dorsal horn. Methods: Twenty-first-day Spinal cord astrocytes from T11 to L6 spinal cord of newly born Wistar rats were cultured in primary culture. The cells were randomly divided into control group (C group), glutamate group (G group) and ketamine group (K group), the remaining three groups were glutamic acid + ketamine group (labeled as GK1 ~ GK3 group) After cultured for 30 minutes, the cells were harvested for detection of [Ca2 +] i, and then cultured for 48 hours to detect the apoptosis rate of astrocytes. Results: Compared with group C, there were a large number of apoptosis in G group (P <0.01) and a significant increase in [Ca2 +] i (P <0.01). Compared with G group, the apoptosis rate and the ratio of [Ca2 +] i (P <0.05). The apoptosis rate and [Ca2 +] i in GK3 group were significantly decreased (P <0.01). Conclusion: Moderate Ketamine significantly inhibited glutamate-induced apoptosis of astrocytes by inhibiting intracellular calcium overload.