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目的探讨微波辐射对大鼠大脑皮质突触体及神经递质释放影响。方法采用30和50mW/cm2微波对大鼠大脑皮质突触体进行辐射;采用流式细胞仪和电子自旋共振仪检测辐射后突触体内Ca2+浓度和膜蛋白流动性;高效液相色谱仪和分光光度法检测氨基酸和乙酰胆碱递质释放量。结果 30mW/cm2微波辐射后突触体内Ca2+浓度为(36.96±1.01),明显高于对照组(P<0.01);膜蛋白强弱固定化比为(0.278±0.022),高于对照组(P<0.05);突触体谷氨酸、甘氨酸和γ-氨基丁酸释放明显减少(P<0.05或P<0.01);50mW/cm2微波辐射后,突触体天门冬氨酸、甘氨酸和γ-氨基丁酸释放明显增加(P<0.05或P<0.01);辐射后突触体乙酰胆碱释放明显增加(P<0.01)。结论微波辐射可引起大脑皮质突触体结构和功能损伤,氨基酸和乙酰胆碱递质释放量发生变化。
Objective To investigate the effect of microwave radiation on synaptosomes and neurotransmitter release in rat cerebral cortex. Methods The synaptosomes of rat cerebral cortex were irradiated by microwave at 30 and 50 mW / cm2. The concentration of Ca2 + and the mobility of membrane protein in synaptosomes were detected by flow cytometry and electron spin resonance. HPLC and Spectrophotometric determination of amino acid and acetylcholine release. Results The concentration of Ca2 + in the synaptosome was (36.96 ± 1.01) after microwave irradiation at 30mW / cm2, which was significantly higher than that in the control group (P <0.01). The immobilization ratio of membrane protein was (0.278 ± 0.022) <0.05). The release of glutamate, glycine and γ-aminobutyric acid in synaptosomes decreased significantly (P <0.05 or P <0.01). After the microwave irradiation at 50mW / cm2, the synaptosomal aspartate, glycine and γ- The release of aminobutyric acid increased significantly (P <0.05 or P <0.01). The release of acetylcholine increased significantly after irradiation (P <0.01). CONCLUSION: Microwave radiation can cause damage to synaptic structure and function of cerebral cortex, and the amount of amino acid and acetylcholine release changes.