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目的比较激素注射间隔时间差异对昆明系小白鼠(KM小鼠)超数排卵的影响以及KM小鼠和ICR小鼠2-细胞胚胎的体外发育能力。方法29只KM小鼠随机分成两组,分别间隔48h和72h注射10 IU的孕马血清(PMSG)和尿绒毛膜促性腺激素(HCG),比较获取2-细胞胚胎数以及其体外发育能力的差异;分7次同时常规超排KM小鼠和ICR小鼠,体外培养2-细胞胚胎,记录囊胚形成数。结果48h超排卵组获取2-细胞期小鼠胚胎数目显著多于72h超排卵组(P<0.01),但前者的囊胚形成率却显著低于后者,两者间差异有统计学意义(P<0.05)。KM小鼠和ICR小鼠2-细胞胚胎的囊胚形成率间无显著差异(P>0.05)。结论间隔48h超排KM小鼠可获取较多胚胎,但间隔72h超排所获2-细胞期小鼠胚胎体外发育潜能好;小鼠品系不影响2-细胞胚胎的体外培养结果。
Objective To compare the effects of hormone injection intervals on superovulation in Kunming mice (KM mice) and in vitro development of KM mice and ICR mice 2-cell embryos. Methods Twenty-nine KM mice were randomly divided into two groups. The pregnant rats were injected with 10 IU of pregnant mare serum (PMSG) and urine chorionic gonadotropin (HCG) at intervals of 48h and 72h respectively. The number of 2-cell embryos and their in vitro development ability Differences; 7 times at the same time conventional superovulation KM mice and ICR mice, 2-cell embryos were cultured in vitro, recording the number of blastocysts. Results The number of 2-cell mouse embryos obtained in 48h super-ovulation group was significantly higher than that of the 72h superovulation group (P <0.01), but the blastocyst formation rate of the former was significantly lower than the latter (P <0.01) P <0.05). There was no significant difference in blastocyst formation between KM mice and ICR mice (P> 0.05). Conclusion More embryos can be obtained from KM mice with 48h intervals, but the embryonic development of 2-cell stage embryos obtained after 72h interval is good. The mouse strains do not affect the in vitro culture of 2-cell embryos.