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建立了一种用稀土离子Sm~(3+)为荧光探针,吐温80为增敏剂测定磺胺氯吡嗪钠的方法。在p H 3.4的B-R缓冲溶液中,Sm~(3+)-吐温80在E_x=260 nm,E_m=378 nm处出现明显的荧光峰,磺胺氯吡嗪钠的加入使得上述荧光峰发生猝灭,且体系的荧光强度猝灭值与磺胺氯吡嗪钠的浓度呈线性关系。基于此建立一种测定磺胺氯吡嗪钠的方法,并对测试条件进行优化选择。在最优条件下,体系的荧光猝灭强度与磺胺氯吡嗪钠质量浓度在0.4~10.0μg/m L范围内呈线性关系,检出限为5.03 ng/m L,并考察了共存物质的干扰情况。方法可用于实际样品及模拟样品中磺胺氯吡嗪钠的测定。
A method was established for the determination of sulfadiazine sodium with the Sm (3+) as fluorescent probe and Tween 80 as sensitizer. Sm (3 +) - Tween 80 showed obvious fluorescence peak at E_x = 260 nm and E_m = 378 nm in BR buffer solution of p H 3.4. The addition of sulfadiazine sodium caused the above fluorescence peak to be abruptly quenched Off, and the quenching value of the fluorescence intensity of the system and the concentration of sulfadiazine sodium showed a linear relationship. Based on this, a method for the determination of sulfasalazine sodium was established, and the test conditions were optimized. Under optimal conditions, the fluorescence quenching intensity of the system was linear with the concentration of sulfamerazine in the concentration range of 0.4 ~ 10.0μg / m L with a detection limit of 5.03 ng / m L. The coexistence of Interference situation. The method can be used for the determination of sulfadiazine sodium in actual samples and in simulated samples.