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目的 研究重复高 +GZ应激对大鼠心肌超微结构的影响 ,建立高 +GZ致心肌损伤的动物实验模型。 方法 2 4只雄性 Wistar大鼠随机分为实验 +1GZ组、+10 GZ组与正常对照组 ,每组 8只。+1GZ 组 :+1GZ 暴露 5 m in/d,3d/周 ,共 3周 ;+10 GZ组 :+10 GZ暴露 30 s/次 ,5次 /d,3d/周 ,共 3周 ;对照组不受加速度作用。实验组大鼠于末次 +GZ实验后次日断头处死 ,迅速从每鼠左室心尖部切取 5小块心肌组织 ,常规透射电镜制样、观察。 结果 +1GZ组心肌结构无明显病变 ,与对照组接近。+10 GZ组心肌细胞基质水肿 ,肌原纤维离散 ,线粒体变性 ,闰盘结构解离。间质血管内皮水肿 ,血管腔内血小板聚集、附壁。 结论 重复高 +GZ应激可致明显的大鼠心肌结构损伤
Objective To study the effects of repeated high + GZ stress on myocardial ultrastructure in rats and to establish an animal model of myocardial injury induced by high + GZ. Methods 24 male Wistar rats were randomly divided into experimental + 1GZ group, +10 GZ group and normal control group, with 8 rats in each group. + 1GZ group: + 1GZ exposure 5 mins / d, 3d / week for 3 weeks; +10 GZ group: +10 GZ exposure 30s / time, 5 times / d, 3d / Not affected by acceleration. Rats in the experimental group were sacrificed on the next day after the last GZ experiment, and 5 small pieces of myocardial tissue were rapidly dissected from the left ventricular apical portion of each rat. Conventional transmission electron microscopy samples were taken and observed. Results + 1GZ group, no significant myocardial structure, close to the control group. +10 GZ group of myocardial cell matrix edema, myofibril dispersion, mitochondrial degeneration, intercalated disc structure dissociation. Interstitial vascular endothelial edema, intraluminal platelet aggregation, attachment. Conclusion Repeated high + GZ stress can cause obvious myocardial structural damage in rats