The degradation of plastic debris may result in the generation of nanoplastics (NPs).Their high specific surface area for the sorption of organic pollutions and toxic heavy metals and possible transfer between organisms at different nutrient levels make the study of NPs an urgent priority.However,there is very limited understanding on the occurrence,distribution,abundant,and fate of NPs in the environment,partially due to the lack of suitable techniques for the separation and identification of NPs from complex environmental matrices.In this review,we first overviewed the state-of-the-art methods for the extraction,separation,identification and quantification of NPs in the environment.Some of them have been successfully applied for the field determination of NPs,while some are borrowed from the detection of microplastics or engineered nanomaterials.Then the possible fate and transport of NPs in the environment are thoroughly described.Although great efforts have been made during the recent years,large knowledge gaps still exist,such as the relatively high detection limit of existing method failing to detect ultralow masses of NPs in the environment,and spherical polystyrene NP models failing to represent the various compositions of NPs with different irregular shapes,which needs further investigation.