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用一种新的细胞培养技术,选用人肺腺癌细胞及人胚肺成纤维细胞,将两者按一定顺序定位培养在同一容器内.用原位计数法分别测定两种细胞在12、36、60、84小时培养的细胞数,计算两种细胞在不同时相的增殖率.观察人肺腺癌细胞和成纤维细胞的相互影响.结果显示:癌细胞在12～36小时期间可刺激成纤维细胞的生长;在36～60小时和60～84小时则抑制肺成纤维细胞的生长.成纤维细胞在60～84小时期间显示对癌细胞的生长起抑制作用.
Using a new cell culture technique, human lung adenocarcinoma cells and human embryonic lung fibroblasts were selected and cultured in the same container in a certain order. Two cells were determined by in situ counting method. 12, 36 The number of cells cultured at 60 and 84 hours was used to calculate the proliferation rate of the two cells at different time phases. The interaction between human lung adenocarcinoma cells and fibroblasts was observed. The results showed that cancer cells can be stimulated during 12 to 36 hours. The growth of fibroblasts inhibited the growth of lung fibroblasts at 36-60 hours and 60-84 hours. Fibroblasts showed inhibition of the growth of cancer cells during 60-84 hours.