论文部分内容阅读
利用基因枪转化法将抗除草剂的bar基因和抗二化螟的SCK(修饰后的CpTI)基因导入到优质粳稻恢复系超优一号中。经对转基因植株进行分子检测,表明外源基因已整合到水稻基因组中;经田间除草剂抗性检测和二化螟接种鉴定,表明转基因稳定株系直到T6仍表现高抗除草剂且没有分离,说明bar基因能稳定遗传并高效表达;转基因恢复系与非转基因恢复系相比,抗虫性有所提高,但提高程度在株系间有差异。经对转基因恢复系所配杂交组合进行除草剂检测和优势测定,表明转基因杂交稻也高抗除草剂,且F1全部正常结实,结实率达80%以上,并具很强的杂种优势,说明亲本抗性基因通过制种已转移到F1中并得到高效表达,同时说明外源基因的导入没有改变原受体的优良性状;经对部分转基因杂交稻进行纯度鉴定,表明秧田喷施除草剂后,本田的不育株率和杂株率明显降低,特别是两系杂交稻效果更明显。
The bar gene of herbicide resistance and the SCK (modified CpTI) gene of resistance to Chilo suppressalis were introduced into the super-good japonica rice restorer line by gene gun transformation. The molecular analysis of the transgenic plants showed that the foreign gene has been integrated into the genome of rice. After the field herbicide resistance test and the identification of the rice stem borer inoculation, the stable transgenic lines showed no herbicide resistance until T6, The results showed that the bar gene could be stably inherited and highly expressed. Compared with the non-transgenic restorer lines, the transgenic restorer lines showed an increase in insect resistance, but the degree of improvement was different among the lines. The results showed that the transgenic hybrid rice was also highly resistant to herbicides, and all of F1 were normal and strong, the seed setting rate was more than 80%, and had a strong heterosis, indicating that the parents The resistance gene was transferred to F1 by seed production and was highly expressed. At the same time, the introduction of exogenous gene did not change the excellent traits of the original receptor. The purity of some transgenic hybrid rice was identified, indicating that after the herbicide was sprayed on the seedling, Honda plant rate of infertility and hybrid plants significantly reduced, especially two-line hybrid rice effect is more obvious.