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目的:探讨穿心莲内酯(AG)衍生物炎琥宁(YHN)对大鼠体内白念珠菌生物膜的影响。方法:构建白念珠菌生物膜大鼠皮下导管模型,采用YHN(40,20,10,5,2.5 mg·kg-1)腹腔注射治疗,设氟康唑(FLC)阳性对照组(80 mg·kg-1),7d后计数导管的菌落形成单位(CFU),XTT代谢法评估YHN对体外白念珠菌生物膜的影响;扫描电镜(SEM)观察YHN干预大鼠体内生物膜的形态变化;采用实时荧光定量PCR法检测白念珠菌黏附相关基因ALS1,ALS3,HWP1,EAP1和MP65的表达量。结果:YHN组导管片上CFU明显少于空白组;XTT代谢活性也显示YHN组低于空白组,且呈剂量依赖性;扫描电镜照片显示YHN能显著减少白念珠菌对大鼠体内导管的黏附;qRT-PCR结果显示,YHN可下调ALS1,ALS3,HWP1,EAP1和MP65的表达量。结论:YHN能抑制大鼠体内白念珠菌生物膜。
AIM: To investigate the effects of YHN, an orrographolide (AG) derivative, on C. albicans biofilm in rats. Methods: A subcutaneous catheter model of C. albicans biofilm was established. The rats were injected intraperitoneally with YHN (40, 20, 10, 5 and 2.5 mg · kg -1), and the positive control group (FLC) kg-1). After 7 days, the colony forming unit (CFU) of the catheter was counted. The effect of YHN on Candida albicans biofilm in vitro was evaluated by XTT method. The morphological changes of the biofilm in YHN-treated rats were observed by scanning electron microscope (SEM) Real-time fluorescence quantitative PCR was used to detect the expression of ALS1, ALS3, HWP1, EAP1 and MP65 in Candida albicans. Results: The CFU of YHN group was significantly less than that of the blank group. The metabolic activity of XTT also showed that the YHN group was lower than the blank group and in a dose-dependent manner. Scanning electron microscopy showed that YHN could significantly reduce the adhesion of C. albicans to the catheter in rats. qRT-PCR results showed that YHN could down-regulate the expression of ALS1, ALS3, HWP1, EAP1 and MP65. Conclusion: YHN can inhibit C. albicans biofilm in rats.