论文部分内容阅读
目的:研究经血宁胶囊对大鼠药物性流产子宫出血的影响,并进行醇提物和水提物(原剂型)平行对比研究,探究两者止血作用的差异。方法:建立药物性流产大鼠子宫出血模型,观察经血宁胶囊对该模型大鼠子宫出血的影响;采用ELISA法测定大鼠血清前列腺素(PGF2a)及大鼠血清血管紧张素Ⅱ(AngⅡ)水平。结果:经血宁胶囊可减少米非司酮+米索前列醇所致的药物性流产大鼠子宫出血的总量,用药组与模型组比较有显著性差异(P<0.05),随着时间的推移每天子宫出血量逐渐减少,经血宁胶囊高、中、低剂量和原剂型组曲线下降幅度依次减小,说明经血宁胶囊减少子宫出血有较好的作用,而且醇提物优于原剂型。经血宁胶囊可增加药物性流产大鼠血清AngⅡ、PGF2a含量,与模型组比较有显著性差异(P<0.05或P<0.01)。结论:经血宁胶囊对药物性流产大鼠宫血有较好的止血作用,表明其止血作用机制之一可能与增加PGF2a和AngⅡ含量有关。
Objective: To study the effect of jingxuening capsule on uterine bleeding in rats induced by medical abortion, and to compare the differences of hemostasis between ethanol extract and water extract (original dosage form). Methods: The model of uterine bleeding in drug-induced abortion rats was established to observe the effect of Jingxuening Capsule on the uterine bleeding in the model rats. The level of serum prostaglandin (PGF2a) and the level of serum angiotensin Ⅱ . Results: The Jingxuening Capsule can reduce the total amount of uterine bleeding in medicated abortion rats induced by mifepristone plus misoprostol. There is significant difference between the medication group and the model group (P <0.05) The daily uterine bleeding gradually decreased, and the decreasing range of high, middle and low doses of Jingxuening Capsules and the curve of the original dosage form decreased in turn, indicating that Jingxuening Capsule has a better effect of reducing uterine bleeding and the alcohol extract is better than the original dosage form. Jingxuening Capsule can increase serum AngⅡ and PGF2a levels in drug-induced abortion rats compared with model group (P <0.05 or P <0.01). Conclusion: Jingxuening Capsule has a good hemostatic effect on uterine blood of rats with medical abortion, indicating that one of the mechanisms of hemostasis might be related to the increase of PGF2a and AngⅡ content.