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为分析东北战区斑点热立克次体(SFGR)的基因型,应用 SFGR 的 rOmp A 基因的 PCP/SSCP分析了东北战区 SFGR 分离株 HL-54、HL-36株的基因型,并与国际标准株及国内其它分离株作了比较。结果显示 HL-54株和 HL-36株二者相同,具有独特的 SSCP 图谱,与国际标准株及国内其它分离株有明显差异。结论:我国 SFGR 分离株存在2个种,即西伯利亚立克次体和斑点热群新种——黑龙江立克次体。PCR/SSCP 分析立克次体型别具有特异、简便、省时等特点。
In order to analyze genotypes of SFGR in Northeast Theater, the genotypes of SFGR isolates HL-54 and HL-36 in Northeast Theater were analyzed by PCP / SSCP of SFGR rOmp A gene and compared with the international standard Strains and other domestic isolates were compared. The results showed that both HL-54 and HL-36 strains were identical and had unique SSCP patterns, which were significantly different from those of international standard strains and other domestic isolates. Conclusion: There are 2 species of SFGR isolates in our country, namely, Siberian rickettsia and speckle fever group - Heilongjiang Rickettsia. PCR / SSCP analysis Rickettsia type is specific, simple, time-saving and so on.