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目的 观察血管紧张素原 (AGT)及血管紧张素Ⅱ 1型受体 (AT1 )蛋白表达改变是否参与模拟失重所致大鼠不同部位动脉血管的分化性适应过程。 方法 以尾部悬吊大鼠模型模拟失重对动脉血管的影响。用Western印迹分析比较 4周模拟失重 (SUS)组与同步对照 (CON)组大鼠基底动脉、颈总动脉、股动脉和肠系膜动脉组织AGT及AT1 的蛋白表达变化。 结果 与CON组相比 ,SUS组大鼠基底动脉组织AGT的表达显著增高 (P <0 .0 5 ) ,在颈总动脉组织呈增高趋势 ,而在股动脉与肠系膜动脉组织均呈降低趋势 ,但差别皆未达到显著水平。与CON组相比 ,SUS组大鼠基底动脉组织AT1 的表达显著增高 (P <0 .0 5 ) ,在颈总动脉组织未见显著变化 ,而在股动脉和肠系膜动脉组织则均显著降低 (P <0 .0 5 )。 结论 模拟失重可引起大鼠脑动脉与后身动脉血管组织的AGT和AT1 蛋白表达发生增高与降低的分化性改变 ,提示血管组织的局部肾素 血管紧张素系统在失重引起的动脉血管分化性适应中可能发挥关键性的调控作用。
Objective To investigate whether changes in the expression of angiotensinogen (AGT) and angiotensin Ⅱ type 1 receptor (AT1) are involved in the differential adaptation of arterial blood vessels in different parts of rats induced by simulated weightlessness. Methods The rat model of tail suspension was used to simulate the effects of weightlessness on arterial blood vessels. The protein expression of AGT and AT1 in the basilar artery, common carotid artery, mesenteric artery and mesenteric artery of rats in 4-week-simulated (SUS) and synchronous (CON) groups were compared by Western blot analysis. Results Compared with CON group, the expression of AGT in basilar artery of SUS rats increased significantly (P <0.05), increased in the common carotid artery, and decreased in the femoral artery and mesenteric artery, However, the differences have not reached a significant level. Compared with CON group, the expression of AT1 in basilar artery of SUS rats was significantly increased (P <0.05), but there was no significant change in common carotid artery, but significantly decreased in femoral and mesenteric artery P <0. 05). Conclusion Simulated weightlessness can induce the differentiation of AGT and AT1 protein in the cerebral arteries and the posterior body of arteries in rats. It is suggested that the local renin-angiotensin system in vascular tissues is adaptive to the arterial differentiation induced by weightlessness May play a key regulatory role.