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目的探讨补体调节蛋白CD46作为一种新的T细胞共刺激分子参与T细胞活化的作用机制。方法磁珠分离纯化人外周血中CD4+T细胞,在加入或未加入外源性IL-2情况下,采用CD3/CD46或CD3/CD28刺激培养。ELISA方法测定培养上清液中IL-10、IFN-γ及IL-4的浓度,用氚标记胸腺嘧啶核苷(3H-TdR)掺入法测定细胞增殖速率。结果在加入外源性IL-2时,CD3/CD46或CD3/CD28刺激CD4+T细胞分泌IL-10水平较缺乏IL-2时明显增加。在中等浓度CD3刺激下,以CD46为协同刺激分子时,CD4+T细胞分泌IL-10水平较以CD28为协同刺激分子时水平升高。以CD46为协同刺激分子时,CD4+T细胞分泌IFN-γ水平降低,两者均无IL-4分泌。在CD3/CD46刺激下,CD4+T细胞增殖能力较CD3/CD28刺激下显著降低。结论 CD46作为一种新的T细胞共刺激分子能够刺激T细胞活化,并导致IL-10分泌增加。
Objective To investigate the role of complement regulatory protein CD46 as a novel T cell costimulatory molecule in T cell activation. Methods CD4 + T cells were isolated and purified from human peripheral blood by magnetic beads. The cells were stimulated with CD3 / CD46 or CD3 / CD28 with or without exogenous IL-2. The concentrations of IL-10, IFN-γ and IL-4 in the culture supernatant were measured by ELISA. The cell proliferation rate was determined by tritiated thymidine incorporation (3H-TdR). Results When exogenous IL-2 was added, the level of IL-10 secreted by CD4 + T cells stimulated by CD3 / CD46 or CD3 / CD28 was significantly increased compared with that of IL-2. When stimulated with CD46 at a moderate concentration of CD3, the level of IL-10 secreted by CD4 + T cells was higher than that when CD28 was a costimulatory molecule. When CD46 is a costimulatory molecule, the level of IFN-γ secreted by CD4 + T cells is decreased, and neither of them has IL-4 secretion. Under the stimulation of CD3 / CD46, the proliferation of CD4 + T cells was significantly lower than that of CD3 / CD28 stimulation. Conclusion CD46 as a new T-cell costimulatory molecule can stimulate T cell activation and lead to an increase of IL-10 secretion.