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一、灭菌料筒体积较大,采用高压灭菌时往往效率低,还易发生胀筒等弊病,大生产只能采用常压灭菌。夏季气温高,为了防止培养料泛酸,应清晨配料,随后集中劳力填料,填毕即灭菌,切不可延至午后,以减少灭菌前微生物自繁生物量。料筒搬入灭菌锅内呈“井”字型堆垛,每堆堆5—6层,然后再置木栅格支架,再继续堆垛,至锅顶。不少初学者为追求堆放筒数,没置活动栅格,也没采用“井”字型堆垛,结果锅底层料筒受上层重力压迫,变成近方形,使灭菌过程产生热蒸气在锅内
First, the sterilizing cylinder volume larger, often low efficiency when using autoclave, but also prone to bulging tube and other ills, large-scale production can only be used under atmospheric pressure sterilization. Summer high temperatures, in order to prevent the pantothenic acid culture, ingredients should be early in the morning, followed by the concentration of labor and stuffing, sterilization is completed at the filling, must not be delayed until noon, in order to reduce the microbial biomass before sterilization. The cartridge was moved into the sterilization pot was “well” font stacking, 5-6 layers of each pile, and then placed wooden racks, and then continue stacking, to the top of the pan. Many beginners for the pursuit of stacking cylinder, no activity raster, nor the use of “well” font stacking, the bottom of the pot under the pressure of gravity by the upper layer into a nearly square, the sterilization process to generate hot steam at Pot