目的：探讨Ｔｕｆｔｓｉｎ激活巨噬细胞杀瘤及其机制。方法：巨噬细胞与肿瘤细胞共培养，细胞毒效应通过ＭＴＴ比色法检测，培养上清ＮＯ浓度采用ＮＯ试剂盒检测。结果：Ｔｕｆｔｓｉｎ呈剂量依赖性增强巨噬细胞细胞毒活性及ＮＯ水平，Ｌ精氨酸进一步增强上述效应，而Ｎ硝基Ｌ精氨酸作用相反。结论：Ｔｕｆｔｓｉｎ能激活巨噬细胞并诱导ＮＯ合成与释放，进而通过ＮＯ产生杀瘤效应。 Objective: To investigate Tuftsin-activated macrophage tumorigenesis and its mechanism. Methods: Macrophages were co-cultured with tumor cells. The cytotoxicity effect was detected by MTT colorimetric assay. The concentration of NO in culture supernatants was detected by NO kit. RESULTS: Tuftsin enhanced the macrophage cytotoxicity and NO levels in a dose-dependent manner. L-arginine further enhanced the above-mentioned effects, whereas N-nitro-L arginine had the opposite effect. CONCLUSION: Tuftsin can activate macrophages and induce the synthesis and release of NO, which in turn produces a tumoricidal effect through NO.