论文部分内容阅读
对象年龄11个月～20岁的肾脏病患者36例(男17、女19),包括SLE1例,IgA肾病7例,过敏性紫癜性肾炎1例,膜性肾病3例,膜性增殖性肾炎2例,增殖性肾炎1例,遗传性肾炎3例,急性肾炎4例,特发性肾病综合征8例,局灶性肾小球硬化症1例,先天性肾病综合征1侧,体位性蛋白尿2例,尿路感染2例。根据需要随访,最长时间两年。方法将一次新鲜尿用纤维膜流水透析后,再用聚乙二醇(分子量20000)把尿液从10倍浓缩到100倍。取此浓缩标本加入人补体成分C_(3c)定量用的免疫扩散板抗原孔中,室温下静置48小时后,测定所形成的沉淀环直径,与标准血清制成的标定曲线比较,即求出和该直径沉淀环相对应的蛋白浓度。
Thirty-six patients with kidney disease (male 17, female 19) aged 11 months to 20 years old included 1 case of SLE, 7 cases of IgA nephropathy, 1 case of allergic purpura nephritis, 3 cases of membranous nephropathy, 2 cases, 1 case of proliferative glomerulonephritis, 3 cases of hereditary nephritis, 4 cases of acute nephritis, 8 cases of idiopathic nephrotic syndrome, 1 case of focal glomerulosclerosis, 1 case of congenital nephrotic syndrome, 2 cases of proteinuria, 2 cases of urinary tract infection. According to the need to follow up, up to two years. Methods Once a fresh urine was dialyzed against a fiber membrane, the urine was concentrated from 10 times to 100 times with polyethylene glycol (molecular weight 20,000). Take this concentrated sample was added to the human complement component C_ (3c) quantitative immunodiffusion plate antigen wells, standing at room temperature for 48 hours after the determination of the diameter of the precipitation ring formed, compared with the standard curve made of serum calibration curve The protein concentration corresponding to the diameter of the precipitation ring.