金鱼(Carassius auratus)鳃对颗粒吸附态铅的吸收

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研究了金鱼 (Carassiusauratus)鳃在悬浮水铝矿吸附态Pb暴露实验中对Pb吸收过程 .结果表明 ,在保持水中游离态Pb浓度不变 ( 0 1mg·L-1)、吸附态Pb浓度增加的条件下 ,鳃中Pb、Al浓度均随暴露浓度增加而增加 ,血液中Pb浓度随鳃中Pb浓度的增加率 (直线斜率 0 171)低于溶解态Pb暴露实验的增加率 (直线斜率0 3 4 3 ) ,附着在鳃上的部分Pb未转移至血液中 ,鳃中Pb/Al比较水相Pb/Al比 ( 0 0 0 3 9)高 1~ 2个数量级 ,并且在组织切片中观察到积聚在粘液上的矿物颗粒 ,从而证实了颗粒态Pb对鱼鳃吸收的生物有效性 .金鱼鳃吸收颗粒态Pb的主要机理是 :流经鳃丝表面的悬浮颗粒物吸附态Pb在鳃表面解离并被动扩散或载体转运穿过鳃部细胞进入血液循环 ,颗粒物在鳃丝表面短时间停留后与脱落粘液一起随水流从鳃部排出 The Pb absorption process of gills in the gibbsite of the Carassius auratus was studied in the experiment of Pb adsorption on suspended gibbsite. The results showed that the concentration of Pb in the adsorbed state increased with keeping the concentration of free Pb in the water constant (0 1 mg · L -1) , The concentrations of Pb and Al in the gills increased with the increase of exposure concentration. The increase of Pb concentration in the gill with the increase of Pb concentration in the gill (linear slope 0 171) was lower than that in the dissolved Pb exposure (linear slope 0 3 4 3), Pb attached to the gills was not transferred to the blood, Pb / Al in the gill was 1 to 2 orders of magnitude higher than the Pb / Al ratio (0 0 0 3 9) in the aqueous phase, and accumulation was observed in the tissue sections In the mucus on the mineral particles, thus confirming the bioavailability of particulate Pb absorbed gills.Gold gill absorption of particulate state of the main mechanism of Pb is: suspended particles flowing through the gill surface adsorption of Pb in the gill surface dissociation and Passive diffusion or carrier transport through the gill cells into the blood circulation, particulate matter in the gill filaments stay for a short time with shedding mucus along with water flow from the gills
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