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在还原物质NADPH及硝酸盐还原酶的催化下 ,显微分光光度法测定细胞及其培养液的氮氧化物含量 ,得到满意结果 :Sander灵敏度为 3 8× 10 -6μmoL/cm2 ,变动系数 (CV % ) 3 96 (n =8) ,标准添加回收率 (10 0 1± 4 96 ) % ,浓度 (C)与吸光值 (A)的线性范围为 0 15～ 1 0 0 μmoL/L ,其回归方程为C =- 0 0 18+ 1 0 99A(γ =0 995 8,P <0 0 1) ,在合适的还原条件下 ,酶促的还原效率为 95 9%。本法适用于生物样品中微量氮氧化物的分析
Under the catalysis of reducing substances NADPH and nitrate reductase, the content of nitrogen oxides in the cell and its culture medium was determined by the micro-spectrophotometry. The results were as follows: the sensitivity of Sander was 38 × 10-6μmoL / cm2, the coefficient of variation %) 3 96 (n = 8), the standard recovery was (1001 ± 4 96)%, and the linear range of concentration (C) to absorbance (A) was 0 15 ~ 1 0 0 μmoL / The equation is C = - 0 0 18 + 1 0 99A (γ = 0 995 8, P <0 0 1). Under appropriate reduction conditions, the enzymatic reduction efficiency is 95 9%. This method is suitable for the analysis of trace nitrogen oxides in biological samples