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目的研究半边旗中二萜类有效成分之一6F对肺癌细胞细胞周期及细胞中DNA、RNA和蛋白质合成的影响。方法采用体外培养的人癌细胞株——肺腺癌上皮细胞SPC-A-1作实验对象,以噻唑蓝(MTT)法测定细胞存活率;用细胞光度术(FCM)测定6F对SPC-A-1细胞周期的影响[3H]-TdR、[3H]-UTR和[3H]-Leu参入的方法观察6F对SPC-A-1细胞DNA、RNA和蛋白质合成的影响。结果化合物6F可强烈抑制SPC-A-1细胞的生长,24h的半数抑制浓度IC50为2.34μmol·L-1;细胞周期分析显示6F作用细胞24h后,均能使细胞的G2/M期细胞比例升高,同时G0/G1期比例下降,并呈一定的剂量效应关系,6F对[3H]-TdR、[3H]-UTP和[3H]-Leu参入作用在培养24h后均有明显抑制作用,且呈剂量依赖关系。结论化合物6F明显抑制SPC-A-1细胞的生长,阻断细胞于G2/M期,抑制细胞DNA、RNA和蛋白质的合成。
Objective To study the effect of 6F, one of the effective components of Diterpenoids, on the synthesis of DNA, RNA and protein in lung cancer cell cycle. Methods The human lung adenocarcinoma cell line SPC-A-1 cultured in vitro was used as the experimental object. The cell survival rate was measured by MTT method. The 6F vs. SPC-A was measured by cytometry (FCM). -1 Effect of cell cycle [3H]-TdR, [3H]-UTR and [3H]-Leu incorporation Methods Observe the effect of 6F on DNA, RNA, and protein synthesis in SPC-A-1 cells. Results Compound 6F could strongly inhibit the growth of SPC-A-1 cells. The half-maximal inhibitory concentration (IC50) at 24 h was 2.34 μmol·L-1. Cell cycle analysis showed that 6F-affected cells could make cells G2/M phase cells for 24 h. The proportion increased, while the proportion of G0/G1 phase decreased, and showed a certain dose-effect relationship. 6F had significant inhibitory effect on [3H]-TdR, [3H]-UTP and [3H]-Leu incorporation after 24 h of culture. , and in a dose-dependent manner. Conclusion Compound 6F can significantly inhibit the growth of SPC-A-1 cells, block cells in the G2/M phase, and inhibit the synthesis of DNA, RNA, and protein.