Radiation dose effects on the morphological development of Mi generation pea(Pisum sativum)

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We irradiated pea seeds with neutrons from a 252Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M1 generation peas.We found that in the dose range of 0.51-9.27 Gy,with the increase in neutron-ab-sorbed dose,the morphological development parameters of M1 generation peas at the initial seedling stage showed an obvious trend with three fluctuations.With the develop-ment of pea,this trend gradually weakened.Further anal-ysis and verification showed that the main trend in the M1 generation of pea seeds was an inhibitory effect induced by neutron irradiation and there was a good linear correlation between the inhibitory effect and neutron absorption dose.We successfully demonstrated the background removal of mutant plants and defined morphological development parameters for peas that match the overall development of plants.Our results will positively impact neutron mutation breeding and automatic agriculture.
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