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目的 研究用 4 0 0 0cGy高能电子线一次照射的大白鼠坐骨神经离体节段 (10 .0mm)原位再植后神经再生的可能性。方法 用 31只Wistar雌性大白鼠 ,1只大白鼠坐骨神经放射线照射后即行光镜、电镜观察 ;余 30只随机分成A组 (单纯手术组 )、B组 (放射手术组 )和C组 (正常对照组 ) ,A、B组经过术后 7、14、2 1、2 8、35、4 2、4 9、5 6、6 3和70天 ,C组饲养 1周 ,测定其坐骨神经功能恢复指标 (SFI)、电生理指标 ,进行组织学观察和计量分析。结果 4 0 0 0cGy放射线严重损伤神经组织。A、B两组光镜下和大体所见无明显差异 ,均于术后 8～ 9周呈现神经再生 ,轴索形态良好 ,功能有一定程度恢复 ;两组计量资料比较无显著性差异 (P >0 .10 ) ,除SFI外各项指标均于术后一段时间达到C组均值水平。结论 经 4 0 0 0cGy高能电子线一次照射 10mm大白鼠坐骨神经离体节段原位再植不妨碍神经再生的速度和质量
Objective To investigate the possibility of nerve regeneration after in situ replantation of isolated rat sciatic nerve (10. 0mm) in a single irradiation with 400eGy high energy electron beam. Methods Thirty-one female Wistar rats and one female rat sciatic nerve were exposed to light and electron microscope. The remaining 30 rabbits were randomly divided into group A (surgery alone group), group B (radiation surgery group) and group C Group A, B after 7,14,2 1,2 8,35,4 2,4 9,5 6,6 3 and 70 days, C group feeding for 1 week, measured sciatic nerve function recovery index ( SFI), electrophysiological indicators, histological observation and measurement analysis. Results 4 0 0cGy radiation seriously damaged the nerve tissue. There was no significant difference between groups A and B under light microscope. All of them showed nerve regeneration 8 to 9 weeks after operation. The axonal morphology was good and the function recovered to some extent. There was no significant difference between the two groups (P > 0.10). Except for SFI, all the indexes reached the mean of group C after a period of time. Conclusions The in situ replantation of isolated sciatic nerve segments of 10 mm rat after 4 0 0 cGy high-energy electron beam irradiation does not hinder the speed and quality of nerve regeneration