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目的研究2007-2009年男男性行为人群(MSM)艾滋病病毒-1型(HIV-1)的基因亚型,以监测MSM人群HIV病毒株的最新流行情况。方法采集95例MSM急性HIV-1感染者的外周全血,从外周全血浓缩白细胞中提取脱氧核糖核酸(DNA),用巢式聚合酶链反应(Nested-PCR)对HIV-1的前病毒DNA进行扩增,然后检测并分析PCR扩增产物的核苷酸序列,确定病毒的基因亚型。结果 95例感染者平均年龄为(35.02±9.15)岁,其中20～50岁的青壮年45例(94.74%),未婚51例(53.68%),大专或大学及以上文化程度者30例(占31.58%)。感染者中共发现CRF01_AE亚型、B亚型、BC重组亚型(包括CRF07_BC和CRF08_BC)及C亚型4种亚型。其中CRF01_AE重组亚型56例(占58.95%),B亚型28例(占29.47%),BC重组亚型10例(占10.53%),C亚型1例(1.05%)。结论 MSM人群急性HIV-1感染者年轻化,文化程度普遍偏低。小样本流行病学调查显示,急性HIV-1感染者中有CRF01_AE亚型、B亚型、BC重组亚型及C亚型4种亚型病毒株流行,CRF01_AE亚型为MSM人群的主要亚型。
Objective To study the genotypes of HIV-1 in men who have sex with men (MSM) in 2007-2009 to monitor the recent prevalence of HIV strains in MSM population. Methods Ninety-five peripheral blood mononuclear cells (PBMCs) from HIV-1-infected MSM patients were collected. DNA was extracted from peripheral leukocytes, and nested polymerase chain reaction (Nested-PCR) DNA amplification, and then detect and analyze the PCR amplification of the nucleotide sequence to determine the virus’s genetic subtype. Results The average age of the 95 infected persons was (35.02 ± 9.15) years old, of which 45 (94.74%) were young and middle-aged adults aged 20-50 years, 51 (56.3%) were unmarried, 30 were college or university graduates 31.58%). The co-infected CRF01_AE subtypes, B subtypes, BC recombinant subtypes (including CRF07_BC and CRF08_BC) and C subtypes of four subtypes. There were 56 cases (58.95%) of CRF01_AE, 28 cases (29.47%) of B subtype, 10 cases (10.53%) of BC subtype and 1 case (1.05%) of C subtype. Conclusion The MSM population is younger and has a lower education level. Epidemiological survey of small samples showed that there were four subtypes of CRF01_AE subtypes, B subtypes, BC subtypes and C subtypes among acute HIV-1 infected persons. The CRF01_AE subtypes were the major subtypes of MSM .