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为了研究T细胞淋巴瘤患者骨髓中恶性T细胞(malignantTcell,MTC)的超微结构,用流式细胞术分析13例T细胞淋巴瘤患者骨髓浸润MTC抗原表达,电子显微镜观察MTC的形态结构。结果发现所有患者MTC大小不等,6/13例细胞大小轻度不均,7例显著不均,直径范围在12-28μm之间。所有患者MTC核异染色质少于正常淋巴细胞,核仁范围在2-8μm之间;10/13例细胞核不规则。8/13例MTC胞浆丰富;7/13例细胞表面有丰富的突起或伪足。5/13例Golgi体、分泌泡、致密颗粒和微丝较丰富;8/13例MTC线粒体肿胀明显。结论骨髓MTC体积普遍不均匀增大;细胞核折叠、切迹、扭曲与核旁微丝增加相关。患者MTC表面突起/绒毛与胞浆Golgi体、分泌泡、致密颗粒及中间微丝呈明显同步发育,大部分患者MTC线粒体明显水肿。
To investigate the ultrastructure of malignant T cells (MTC) in the bone marrow of patients with T-cell lymphoma, flow cytometry was used to analyze the expression of MTC antigen in 13 patients with T-cell lymphoma. The morphology of MTC was observed by electron microscopy. The results showed that all patients with MTC size range, 6/13 cases of mild cell size unevenness, 7 cases were significantly uneven diameter range between 12-28μm. All patients with MTC nuclear heterochromatin less than normal lymphocytes, nucleoli in the range of 2-8μm; 10/13 cases of irregular nuclei. 8/13 cases of MTC abundant cytoplasm; 7/13 cases of cell surface rich in protuberance or pseudopodia. 5/13 Golgi bodies, secretory bubbles, dense granules and microfilaments richer; 8/13 MTC mitochondria swelling significantly. Conclusion The volume of bone marrow MTC generally increases nonuniformly. The cell nucleus fold, notch and twist correlate with the increase of nuclear parafascia. In patients with MTC surface protrusions / villi and cytoplasm Golgi body, secretory vesicles, dense particles and the middle of the micro-silk was significantly synchronized development, most patients MTC mitochondria significantly edema.