论文部分内容阅读
以地高辛(DIG)标记的黄毛草莓基因组DNA为探针、凤梨草莓基因组DNA为封阻对黄毛草莓×凤梨草莓杂种F1的五倍体植株根尖染色体进行基因组原位杂交(GISH),结果表明利用GISH可将黄毛草莓染色质从凤梨草莓中检测出来,从而证实了黄毛草莓与凤梨草莓种间杂交的真实性。利用RAPD技术对来自黄毛草莓与凤梨草莓不同品种的2个杂交组合获得的14个株系及其亲本的DNA指纹图谱进行分析。结果表明,不同株系之间在基因组水平上具有高度异质性,以引物S1371和S1405能将黄毛草莓×春霄组合的10个杂交后代株系加以区别,以单一引物S1371、S1380、S1397、S1404、S1405可使黄毛草莓×硕香后代株系之间相互区别。在杂交后代中观察到了亲本黄毛草莓与凤梨草莓不同栽培品种的特征带,从而进一步证实黄毛草莓与凤梨草莓种间杂交后代的真实性。
Genomic DNA of Drosophila melanogaster detected by digoxin (DIG) was used as a probe, genomic DNA of pineapple strawberry was blocked by GISH in the apical chromosome of the pentaploid plants of yellow strawberry × hybrid strawberry. , The results showed that the use of GISH can be detected from the yellow strawberry strawberry pineapple, confirming the authenticity of the cross between yellow strawberry and pineapple strawberry. RAPD was used to analyze the DNA fingerprinting of 14 lines and their parents obtained from two crosses of different cultivars of Rosa mandshurifulou and Pineapple strawberry. The results showed that there were highly heterogeneous genomic levels among different lines. The primers S1371 and S1405 were able to distinguish the 10 hybrid progeny lines from the combination of Rosa mandshurica × Chunxiao. Single primers S1371, S1380 and S1397 , S1404, S1405 can make the difference between each other. The characteristic bands of different cultivars of parent yellow hairy strawberry and pineapple strawberry were observed in hybrid progenies, which further confirmed the authenticity of cross between offspring of yellow-haired strawberry and pineapple strawberry.