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目的观察芹菜根不同部位对四氯化碳所致大鼠急性肝损伤的影响,筛选其护肝作用的有效部位。方法 70只Wister大鼠随机分为7组:模型组、正丁醇组、石油醚组、氯仿组、乙酸乙酯组、阳性组、空白组。其中石油醚组、氯仿组、乙酸乙酯组、正丁醇组分别以1.5g.kg-1灌胃相应部位的药物,模型组与空白组以100g.ml-1用量灌胃生理盐水;阳性组按200mg.kg-1灌胃联苯双酯,共7天。第7天,模型组、阳性组和各给药组按1ml.kg-1腹腔注射CCl4溶液,阴性组注射等体积生理盐水。测定各组大鼠血清门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)。结果乙酸乙酯部位,正丁醇部位均能不同程度地降低肝损伤大鼠的血清ALT、AST水平。结论维药芹菜根护肝作用的有效部位为乙酸乙酯部位,正丁醇部位。
Objective To observe the effects of different parts of celery root on carbon tetrachloride-induced acute liver injury in rats and to screen the effective parts of liver protection. Methods Seventy Wister rats were randomly divided into seven groups: model group, n-butanol group, petroleum ether group, chloroform group, ethyl acetate group, positive group and blank group. The petroleum ether group, the chloroform group, the ethyl acetate group and the n-butanol group were administrated with 1.5g.kg-1 respectively, and the model group and the blank group were administered with 100g.ml-1 dosage of normal saline; the positive Group by 200mg.kg-1 gavage bifendate, a total of 7 days. On the 7th day, the model group, the positive group and each administration group were injected intraperitoneally with 1 ml.kg-1 of CCl4 solution, and the negative group was injected with the same volume of normal saline. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were determined in each group. Results Both ethyl acetate fraction and n-butanol fraction could decrease the levels of serum ALT and AST in rats with liver injury. Conclusion The effective part of the liver root in the root of traditional Chinese medicine is the ethyl acetate fraction and n-butanol fraction.