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采用高效液相色谱－荧光法测定了血浆及尿液中左氧氟沙星的浓度。Nova－PakC18预柱，SymmetryC18分析柱，已腈－0．05mol/L枸橼酸－1mol/L醋酸铵（11．5：77：1）为流动相，激发波长300um，发射波长500um。血中左氧氟沙星回收率为100．5％，RSD为1％。尿中左氧氟沙星回收率为99．0％，RSD为1．6％。最低检测量为8ng。
The concentrations of levofloxacin in plasma and urine were determined by high performance liquid chromatography-fluorescence spectrometry. Nova-PakC18 precolumn, SymmetryC18 analytical column, nitrile-0.05mol / L citric acid-1mol / L ammonium acetate (11.5: 77: 1) as mobile phase with excitation wavelength 300um and emission wavelength 500um. Levofloxacin blood recovery was 100.5%, RSD was 1%. Urinary levofloxacin recovery was 99.0% with a RSD of 1.6%. The minimum detection capacity of 8ng.