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探讨槲皮素对LPS刺激的小胶质细胞炎症因子的下调作用。用不同浓度的槲皮素处理细菌脂多糖(LPS)诱导过的BV2小胶质细胞,观察不同浓度的槲皮素对炎症因子:一氧化氮(NO)、肿瘤坏死因子(TNF-α绿)以及白介素-1β(IL-1β)的抑制效果。槲皮素在10μm、20μm、30μm时可降低炎症因子NO、TNF-α绿以及IL-1β的产生,与LPS组相比只加10μmol/L槲皮素处理:NO下降17.26%,IL-1β下降21.21%,TNF-α绿下降18.93%;20μmol/L槲皮素处理:NO下降45.18%,IL-1β下降35.45%,TNF-α绿下降37.77%;30μmol/L槲皮素处理:NO下降72.59%,IL-1β下降57.59%,TNF-α绿下降62.32%。但只在20μmol/L、30μmol/L槲皮素处理时与LPS组相比NO、TNF-α绿以及IL-1β的下降有统计学差异(p<0.05)。与上述相同与LPS组相比槲皮素为10μmol/L、20μmol/L、30μmol/L时可降低炎症因子TNF-α绿以及IL-1βm RNA的产生,10μmol/L槲皮素处理:IL-1βmRNA下降16.88%,TNF-α绿mRNA下降14.88%;20μmol/L槲皮素处理:IL-1βmRNA下降38.96%,TNF-α绿mRNA下降37.16%;30μmol/L槲皮素处理IL-1βmRNA下降55.49%,TNF-α绿mRNA下降54.38%。但只在20μmol/L、30μmol/L槲皮素处理时TNF-α绿以及IL-1β的mRNA下降有统计学差异(p<0.05)。槲皮素对LPS刺激的小胶质细胞炎症因子有一定的下调作用,其抗炎机制可能与下调NO、TNF-α绿以及IL-1β的产生有关。
To investigate the down-regulation effect of quercetin on LPS-stimulated microglial inflammatory cytokines. BV2 microglial cells induced by bacterial lipopolysaccharide (LPS) were treated with different concentrations of quercetin. The effects of different concentrations of quercetin on inflammatory factors such as nitric oxide (NO), tumor necrosis factor (TNF-αgreen) And the inhibitory effect of interleukin-1β (IL-1β). Quercetin at 10μm, 20μm and 30μm reduced the production of NO, TNF-α and IL-1β. Compared with LPS group, quercetin treatment reduced NO by 17.26% and IL-1β (P <0.05). The level of TNF-α decreased by 21.21% and the level of TNF-α decreased by 18.93%. The treatment with 20μmol / L quercetin reduced NO by 45.18%, IL- 72.59%, IL-1β decreased 57.59%, TNF-α green decreased 62.32%. However, there was a significant difference (p <0.05) in NO, TNF-αgreen and IL-1βin 20μmol / L and 30μmol / L quercetin treatment compared with LPS group. Compared with LPS group, quercetin at 10μmol / L, 20μmol / L and 30μmol / L could reduce the production of inflammatory cytokines such as TNF-α and IL-1βmRNA. 10μmol / 1|ÌmRNA decreased by 16.88% and TNF-|ÁmRNA decreased by 14.88%. When treated with 20|Ìmol / L quercetin, the expression of IL-1|Â mRNA decreased by 38.96% and TNF-|ÁmRNA decreased by 37.16% %, TNF-α green mRNA decreased 54.38%. However, there was a significant difference (p <0.05) in TNF-αgreen and IL-1β mRNA decline only at 20μmol / L and 30μmol / L quercetin treatment. Quercetin has a certain down-regulation effect on LPS-stimulated microglial inflammatory cytokines, and its anti-inflammatory mechanism may be related to the down-regulation of NO, TNF-αgreen and the production of IL-1β.