研究救脑宁注射液对缺氧 /缺糖致神经细胞凋亡的影响。采用人神经母细胞瘤细胞株 (SH SY5H) ,建立缺氧 /缺糖诱导凋亡模型 ,以流式细胞技术和活细胞荧光双染色法观察凋亡的发生及救脑宁注射液抑制细胞凋亡的作用。结果 :缺氧 /缺糖培养能诱导神经细胞凋亡 ,流式细胞仪检测 ,在G1峰前出现显著的凋亡峰 ,凋亡率 42 99%± 7 73%。荧光双染色后 ,荧光显微镜下观察到凋亡时细胞核染色质凝聚。中大剂量救脑宁注射液能降低细胞凋亡率 ,与模型组相比差异显著 (P<0 0 1 ) ,与神经生长因子组相比差异无统计学意义。结论 :救脑宁注射液可抑制缺氧 /缺糖诱导的神经细胞凋亡 ,具有神经细胞保护作用。 To study the effect of Jiunaoning injection on apoptosis of nerve cells induced by hypoxia/glucose deprivation. Human neuroblastoma cell line (SH SY5H) was used to establish a model of apoptosis induced by hypoxia/glucose deficiency. Flow cytometry and live cell fluorescence double staining were used to observe the occurrence of apoptosis and Jiunaoning Injection inhibited cell apoptosis. The role of death. RESULTS: Hypoxia/glucose deprivation could induce apoptosis of neurons. Flow cytometry showed that there was a significant apoptotic peak before G1 peak. The apoptotic rate was 42 99% ± 7 73%. After fluorescent double staining, nuclear chromatin condensation was observed under fluorescence microscopy. Medium and large doses of Jiunaoning injection can reduce the rate of apoptosis, compared with the model group, the difference is significant (P < 0 01), compared with the nerve growth factor group, the difference was not statistically significant. Conclusion: Jiunaoning Injection can inhibit neuronal apoptosis induced by hypoxia/glucose deprivation and has neuroprotective effect.