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从一尾转ｐＭＴｈＧＨ基因红鲤Ｆ４代仔鱼基因组内回收并克隆了５０个转植基因（ｔｒａｎｓｇｅｎｅ），对其中３３个进行了分析．根据双酶切结果，可将这些回收基因分为４种类型．用５种内切酶构建了这 ４种类型回收基因的限制性内切酶图谱，第 １种类型（６个，占 １８％）与建立原代转基因鱼所用的外源基因相同，而其他３种类型回收基因的分子量大小、酶切位点的种类、数量、位置都迥异于转移前的结构，意味着大多数转植基因顺序发生了缺失和重排．Ｓｏｕｔｈｅｒｎ杂交进一步证明了这一结果．对回收基因侧翼进行顺序分析，鉴别了两个相关的整合位点，即鲤鱼β－ａｃｔｉｎ基因的启动子或５’ ＵＴＲ位点，以及与小鼠 ＲＴＫ ｍＲＮＡ同源的鲤鱼基因顺序位点。
Fifty transgene transcripts were recovered and cloned from the genome of F4 generation larvae transferred from one pMThGH gene to another and 33 of them were analyzed. According to double digestion results, these recovered genes can be divided into four types. The restriction endonuclease map of these 4 types of reclaimed genes was constructed using 5 endonucleases. The first type (6, 18%) was identical to the exogenous gene used to establish the primary transgenic fish, whereas the other 3 The molecular weight of the type of reclaimed gene, the type, number and location of restriction sites are all very different from those before the transfer, which means that most of the transgene sequences are deleted and rearranged. Southern hybridization further confirmed this result. Sequence analysis of the recovered gene flanks identified two related integration sites, namely the promoter or 5 ’UTR site of the carp beta-actin gene and the carp gene sequence site homologous to the mouse RTK mRNA.