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目的:研究β榄香烯对BIU87 细胞的抗癌作用机制。方法:采用细胞培养、电子显微镜、细胞DNA凝胶电泳和荧光探测等技术,探讨β榄香烯抗癌作用机制。结果:①β榄香烯作用瘤细胞后,光镜观察发现瘤细胞外形逐渐变小变圆,同时明显抑制瘤细胞的生长;电镜观察发现瘤细胞的细胞核染色质密度增高,呈半月形,并凝聚在核膜周围。②β榄香烯作用瘤细胞后,提取瘤细胞DNA,经β榄香烯作用后的样品呈现DNA梯带现象,而对照组则为阴性。③β榄香烯作用瘤细胞后,以Fura 2AM 负载,随着药物浓度的升高,瘤细胞内游离Ca2+ 浓度明显升高,呈明显的量效关系( P<0.01)。结论:β榄香烯诱发BIU87 细胞所出现凋亡的早期变化,可能是其抗肿瘤作用机制的一个重要方面
Objective: To study the anticancer mechanism of β-elemene on BIU87 cells. METHODS: Cell-culture, electron microscopy, cell DNA gel electrophoresis and fluorescence detection were used to investigate the anticancer mechanism of β-elemene. RESULTS: After 1β-elemene acted on the tumor cells, the shape of the tumor cells gradually became smaller and rounder, and the growth of the tumor cells was significantly inhibited by light microscopy. The electron microscopy showed that the nuclear chromatin density of the tumor cells increased, showing a half-moon shape, and Convergent around the nuclear membrane. After 2β-elemene acted on the tumor cells, the DNA of the tumor cells was extracted, and the DNA ladder phenomenon was exhibited in the samples treated with β-elemene, but it was negative in the control group. After 3β-elemene acted on tumor cells, with the increase of drug concentration, the concentration of free Ca2+ in tumor cells increased significantly with Fura 2AM loading, showing a significant dose-effect relationship (P<0.01). Conclusion: The early changes in apoptosis induced by β-elemene in BIU-87 cells may be an important aspect of its anti-tumor mechanism.