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The mosaic structure of landscape of the central area of Shanghai Metropolis is studied by quantitative methods of landscape ecology based on Remote Sensing (RS) and Geographic Information System (GIS) in this pa-per. Firstly, landscapes are classified into eight categories: residential quarter, industrial quarter, road, other urban landscape, farmland, village and small town, on-building area, river and other water bodies (such as lake, etc.). Sec-ondly, a GIS is designed and set up based on the remote sensing data and field investigation, and a digital map of landscape mosaic is made. Then the indexes of diversity, dominance, fragmentation and isolation, and fractal dimen-sion of each type of landscape in different periods are calculated by using spatial analysis method of GIS. With refer-ence to the calculated results, a series of relative issues are discussed.