[目的]观察无花果叶提取物体外抗单纯疱疹病毒-1型的作用。[方法]采用原代兔肾细胞培养技术,以无环鸟苷为阳性对照,通过观察细胞病变检测无花果叶水体物、醇提物对病毒的直接作用、治疗作用和预防作用。[结果]无花果叶水提物、醇提物的半数毒性浓度(TC50)分别为184.4 mg/ml和485.3 mg/ml。无花果叶提取物体外对HSV-1具有明显的抑制和杀灭作用。[结论]无花果叶提取物体外具有明显的抗HSV-1的作用。 [Objective] To observe the effect of fig leaf extract against herpes simplex virus-1. [Methods] The primary rabbit kidney cell culture technique was used. Acyclovir was used as a positive control, and the direct effect, therapeutic effect and preventive effect of fig leaf water bodies and alcohol extracts on the virus were observed by observing the cytopathological changes. [Results] The half toxic concentrations (TC50) of the aqueous extracts and alcohol extracts of fig leaves were 184.4 mg/ml and 485.3 mg/ml, respectively. The fig leaf extract has obvious inhibition and killing effect on HSV-1. [Conclusion] The fig leaf extract had obvious anti-HSV-1 effect.