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作者采用热酚水法提取了具核梭杆菌ATCC25586的脂多糖(LPS),并用苯酚一氯仿—石油醚(PCP)和电透析对脂多糖进一步纯化,结果得到了高纯度的内毒素脂多糖。其蛋白质和核酸含量分别低于1.5％。氨基葡萄糖含量为81.4μg/mg。总磷含量为30.2μg/mg。用薄层层析对糖成分进行分析发现其多糖区域存在着2—酮—3—脱氧基—D—甘露醇辛酮糖酸(KDO)。鲎试剂凝集最低剂量为3.125pg。
The authors extracted lipopolysaccharide (LPS) from Fusobacterium nucleatum ATCC25586 by hot phenolic water method and purified the lipopolysaccharide with phenol-chloroform-petroleum ether (PCP) and electrodialysis. As a result, high-purity endotoxin lipopolysaccharide was obtained. Its protein and nucleic acid content were less than 1.5%. Glucosamine content was 81.4 μg / mg. The total phosphorus content was 30.2 μg / mg. Analysis of the sugar components by thin layer chromatography revealed the presence of 2-keto-3-deoxy-D-mannitol octocopheroic acid (KDO) in the polysaccharide region.最 The lowest dose of reagent agglutination is 3.125pg.