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目的了解湖南省流感监测地区的流感流行状况及流感毒株的型别分布,分析其流行趋势,为流感防制提供科学依据。方法采集流感样病例(ILI)的咽拭子标本,用传代狗肾细胞(MDCK)进行病毒分离,采用血凝抑制实验(HI)进行流感病毒型别鉴定。分离的毒株再送国家流感中心(NIC)进行复核鉴定。结果全省12家哨点医院3499份ILI咽拭子标本,分离到毒株258株,分离阳性率为7.37%,经NIC最后复核鉴定的结果为:A(H1N1)亚型202株,A(H3N2)亚型11株,B型43株,另有2株送NIC后转阴;流感及ILI暴发疫情病例咽拭子标本179份,分离到流感病毒75株,分离阳性率为41.90%,分型鉴定为A(H1N1)亚型12株,B型62株,1株送国家流感中心后转阴。结论2006年湖南省流感监测地区全年均有流感活动,A(H1N1)亚型、A(H3N2)亚型和B型均能被分离到,A(H1N1)亚型为优势株,而暴发疫情则以B型为主。
Objective To understand the prevalence of influenza in influenza surveillance area in Hunan Province and the type distribution of influenza strains and to analyze their epidemic trends and provide a scientific basis for influenza prevention and control. Methods Throat swab samples of influenza-like illness (ILI) were collected, and virus was isolated by passaged dog kidney cells (MDCK). Influenza type was identified by hemagglutination inhibition test (HI). The isolated strains are sent to the National Influenza Center (NIC) for verification. Results 3499 ILI throat swabs from 12 sentinel hospitals in the province were isolated and 258 strains were isolated. The positive rate of isolation was 7.37%. The results of NIC final check were: 202 strains of A (H1N1), A 11 strains of H3N2 subtype and 43 strains of B type. Another 2 strains were sent to NIC and turned negative. Throat swab specimens of 179 cases of influenza and ILI outbreak were isolated, and 75 strains of influenza virus were isolated. The positive rate was 41.90% Type A was identified as A (H1N1) subtype 12 strains, B type 62 strains, one sent to the national influenza center after the negative. Conclusion Influenza surveillance in Hunan Province in 2006 showed that all influenza A (H1N1), A (H3N2) and B (B) isolates were isolated during the year. Influenza A (H1N1) subtype was predominant and influenza A B-based.