论文部分内容阅读
本实验主要观察并比较了大鼠冷适应前后直肠温度(RT)、血清游离脂肪酸(SFFA)浓度、肩胛间棕色脂肪组织(IBAT)和肝脏cAMP含量的变化及其对去甲肾上腺素(NE)反应性的改变。结果表明:①冷适应28d大鼠在冷环境中RT稳定,NE刺激后RT上升幅度大于常温对照组(P<0.005);②冷适应1d组SFFA升高,冷适应28d组SFFA接近对照组,且对NE刺激无反应,对照组给NE后SFFA与RT一致性升高;③冷适应28d组IBAT的cAMP升高,而肝脏的cAMP含量三组间无显著性差异。NE刺激后,冷适应28d组IBAT和肝脏cAMP均升高,与RT反应一致,而对照组不变。结果提示,在5±3℃适应28d的大鼠已建立冷适应机制,非寒颤产热(NST)容量增加,在冷适应的不同时期,肝脏和IBAT调节NST的机制不同。
In this study, we observed and compared the changes of rectal temperature (RT), serum free fatty acid (SFFA), inter-scapular brown adipose tissue (IBAT) and hepatic cAMP before and after cold acclimation in rats and their effects on norepinephrine Reactivity changes. The results showed that: (1) RT of cold-adapted rats was stable in cold environment for 28 days, and that of RT increased more significantly than that of normal temperature (P <0.005) after NE stimulation; ② SFFA of cold-adapted 1d group was increased and SFFA of cold-adapted 28d group was close to control group, And there was no response to NE stimulation. The consistency of SFFA and RT in control group was increased after NE administration. ③ The cAMP of IBAT in cold-adapted 28-day group was increased, while there was no significant difference in cAMP content in liver between the three groups. After NE stimulation, the cold adapted 28-day IBAT and liver cAMP were increased, consistent with the RT response, while the control group unchanged. The results suggest that cold acclimation (NST) capacity is increased in rats acclimated for 28 days at 5 ± 3 ℃. Different mechanisms of liver and IBAT regulate NST at different periods of cold acclimation.