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目的分析2型糖尿病患者肠道拟类菌属和双歧杆菌属的多样性及其与健康个体的差异,探讨糖尿病与肠道优势菌属的相关性。方法收集2型糖尿病患者和健康志愿者粪样,提取样品中菌群总DNA,利用拟类菌和双歧杆菌16SrRNA种属特异性引物,进行PCR扩增,将产物用变性梯度凝胶电泳分离,获得肠道菌群分子指纹图谱,进行多样性和相似性等特征分析。结果糖尿病组和健康人组样品DGGE图谱多样性指数范围分别是:拟类菌属0.89(0.75～0.95),0.95(0.89～0.97);双歧杆菌属:0.85(0.75～0.94),0.87(0.73～0.93)。组内成对相似性系数累积曲线分析,拟类菌属:小于0.5的Cs值在糖尿病组占到总Cs值个数的85.83%,而健康人组只占到35.71%;双歧杆菌属:小于0.4的Cs值在糖尿病组占到总Cs值个数的90%,健康人组占到总Cs值个数的60.7%。结论糖尿病患者和健康人肠道拟类菌属和双歧杆菌属指纹图呈现个体特异性,糖尿病患者两种菌属多样性与健康个体无显著差异,而糖尿病患者个体之间的相似性较健康个体之间低。
Objective To analyze the diversity of intestinal sib-like and bifidobacteria in patients with type 2 diabetes mellitus and their differences with healthy individuals, and to explore the correlation between diabetes and intestinal dominant bacteria. Methods The fecal samples from patients with type 2 diabetes and healthy volunteers were collected and the total bacterial DNA was extracted from the samples. PCR amplification was performed using the 16S rRNA specific primers of the 16SrRNA and Bifidobacterium species. The products were separated by denaturing gradient gel electrophoresis , Access to intestinal flora molecular fingerprinting, diversity and similarity characteristics such as analysis. Results The range of diversity index of DGGE in samples of diabetic and healthy subjects were: 0.89 (0.75-0.95), 0.95 (0.89-0.97), 0.85 (0.75-0.94), 0.87 (0.73 ~ 0.93). In the group, the cumulative curve analysis of paired similarity coefficient showed that CsA was less than 0.5, accounting for 85.83% of the total Cs value in the diabetes group and only 35.71% of the healthy people; Bifidobacterium: The Cs value less than 0.4 accounted for 90% of the total Cs value in the diabetes group and 60.7% of the total Cs value in the healthy person group. Conclusion The fingerprints of gut bacteria and bifidobacteria in patients with diabetes mellitus and healthy people show individual specificity. There is no significant difference between the two genus of bacteria in diabetes and healthy individuals, while the similarity among individuals with diabetes is healthier Low between individuals.