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目的:探讨大蒜素预处理对在体大鼠心肌缺血/再灌注(I/R)损伤的保护作用、对心肌细胞凋亡的影响及Bcl-2,Bax,NF-κB是否参与抗凋亡作用。方法:建立大鼠在体心肌I/R损伤模型,将48只大鼠随机分为正常对照(Con)组、I/R组和大蒜素预处理(AP)组;各组均测定心肌梗死范围[IS/AAR(%),TTC法]、再灌注后血清肌酸磷酸肌酶同工酶MB(CK-MB)和超氧化物歧化酶(SOD)活性,并应用DNA琼脂糖凝胶电泳及TUNEL法检测各组凋亡指数(AI)、Bcl-2,Bax表达及NF-κB核结合活性。结果:AP组较I/R组IS/AAR(%)[(21.8±1.5)%vs.(44.6±4.6)%,P<0.01],CK-MB[(16.4±1.6)vs.(34.1±1.8)U/L,P<0.01]明显降低,SOD[(2 337±215)vs.(1 219±187)U/mg pro,P<0.01]明显增高。AP组AI较I/R组明显降低[(7.0±1.2)%vs.(4.0±3.0)%,P<0.01]。Bcl-2表达明显增加,Bax表达明显减少,NF-κB核结合活性明显降低。结论:大蒜素有明显抗大鼠心肌I/R损伤作用能与抗心肌细胞凋亡有关,对Bcl-2,Bax及NF-κB表达的调控起重要作用。
Objective: To investigate the protective effect of allicin preconditioning on myocardial ischemia / reperfusion (I / R) injury in vivo and its effect on cardiomyocyte apoptosis and whether Bcl-2, Bax and NF-κB are involved in anti-apoptosis effect. Methods: The rat model of myocardial I / R injury was established. 48 rats were randomly divided into normal control (Con) group, I / R group and allicin preconditioning (AP) group. The myocardial infarct size [IS / AAR (%), TTC method] and serum creatine phosphokinase MB (CK-MB) and superoxide dismutase (SOD) activity after reperfusion were detected by DNA agarose gel electrophoresis and TUNEL Methods Apoptosis index (AI), Bcl-2, Bax expression and NF-κB nuclear binding activity in each group were detected. Results: Compared with I / R group, the AP / AAR scores in the AP group were significantly higher than those in the I / R group (P <0.01) 1.8) U / L, P <0.01]. The SOD level was significantly higher than that in the control group [(2 337 ± 215) vs. (1119 ± 187) U / mg pro, P <0.01] AI in AP group was significantly lower than that in I / R group [(7.0 ± 1.2)% vs (4.0 ± 3.0)%, P <0.01]. Bcl-2 expression was significantly increased, Bax expression was significantly reduced, NF-κB nuclear binding activity was significantly reduced. CONCLUSION: Allicin has obvious anti-myocardial I / R injury effect on cardiomyocyte apoptosis, and plays an important role in regulating the expression of Bcl-2, Bax and NF-κB.